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Excess staple strands were removed from DNA origami blocks by agarose gel purification.
Intact scaffolds were separated from excess staple strands using Amicon Ultra 100K cutoff spin columns (EMD Millipore).
A 200 μL amount of sample solution containing the assembled DNA origami structures and excess staple strands was mixed with 40 μL of 6× loading dye and then loaded into the gel pockets.
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Two cycles removed 99.4%% of all excess staples, and three cycles gave samples that were essentially free of non-integrated staple strands.
When you have all the images printed out, trim off the excess paper, staple the pages together, and flip.
Nanostructure synthesis was carried out by mixing 50 nM M13 scaffold with a 5× excess of staples in TE + 14 mM (DNO), 20 mM (NT), 18 mM (NR), or 8 mM (nanorobot) MgCl2.
Linkers and protein modules fused to the linkers, were at 5 μM, and were incubated 1 h at 24 °C with a 1.5-fold excess of relevant staple.
Cut the corners of the material so there is less excess fabric for stapling, then fold the corners in and secure using 2 staples approximately 1 inch (2.54 cm) apart from each other.
Staple mesh in place with staple gun, cutting excess mesh with wire cutters.
The government, buoyed by a good harvest, which enabled it to avoid cereal imports and even to export excess barley, subsidized 15 consumer staples to ease social discontent.
Crazy outfits, neon, and a definite excess of exposed skin are staples of the 3-day electronic music rager down in Miami.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com