Poly-β-hydroxybutyric acid (PHB) is a natural, biodegradable polymer, which is accumulated as an energy reserve material by a large number of bacteria when, nutrients such as nitrogen or phosphorous sources are available in limiting concentrations in the presence of excess carbon source.
The effect of 10B on SER as an in situ excess charge source is observed.
Under this condition, the excess carbon source is channeled into lipid bodies to form triglycerides (TAGs).
Furthermore, in all these new Chelatococcus isolates, a growth-associated accumulation of intracellular PHB granules was observed when growing in medium with excess carbon source (Ibrahim et al. 2010).
Accumulated under condition of excess carbon source with lacking of other nutrients (Magdouli et al. 2015; Dalal et al. 2013), PHAs serve as intracellular carbon and/or energy storage reserve in various microorganisms (Koller et al. 2011).
Additionally, the annealing process of sucrose coating may offer much higher local temperature to improve the crystallization and volatile the excess Li source to suppress Li2CO3 impurity formation verified by the result of XPS.
This chapter discusses the approach to introduce the genes encoding an omega-3 fatty acid biosynthesis pathway into an oleaginous yeast that synthesizes and stores triglycerides as an energy reserve when starved for nitrogen in the presence of an excess carbon source, such as glucose.
Under C-limited feeding conditions, no excess carbon source was present in the medium.
The high yields of enzymes and growth were generally obtained by using an excess nitrogen source under conditions of carbon limitation.
However, the excess carbon source (D-fructose) was able to maintain the pool of acetyl-CoA for retarded growth and the excess acetyl-CoA was redirected to PHB production at levels below the optimal C/N uptake ratio.
The corresponding Gene Ontology (GO) terms are listed in Additional file 2. A high correlation between the two excess carbon source condition transcriptomes (G and D) was also observed by principal component analysis (PCA), which showed a good correlation of the biological replicates of each condition.
