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Which is a pity, because taken in another light, Flowers' extraordinary tale of excess reads a bit like that Jenny Joseph poem.
This finding indicates that random sampling would be a simple and effective procedure for finding the optimum coverage and best combination of reads for de novo assembly when excess reads are available.
In the absence of a variant, errors should randomly distribute across multiple pools while for a genuine rare variant excess reads of the alternative allele are expected to concentrate in one or several pools.
The BAM files containing the H3K27ac ChIP-seq reads were normalized to the same number of reads per dataset by randomly removing excess reads from the larger BAM files.
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"For a religious leader to question a person's faith is disgraceful," Trump, now seemingly a victim of the Pope's excess, read from a statement he then released.
Finally, we removed sites that occur within a total of 17 Mb of the genome that receive excess read alignment in 1000 Genome Project data (Genovese et al., 2013).
In order to reconstruct the evolutionary origins of those segmental duplications, we used whole-genome shotgun (WGS) sequences from human, chimpanzee, orangutan and macaque to detect regions of excess read depth against the human genome reference assembly (build35) (35).
We analyzed Neandertal segmental duplications by measuring excess read-depth to identify and predict the copy number of duplicated sequences, defined as those with >95% sequence identity (62).
Second, there is an excess of reads that map to HpaII fragments 40 250 bp in length and a deficit of reads mapping to HpaII fragments 0 30 bp in length (Figure 2A).
Within this population, 38% (120/312) exhibited a large excess of reads, from 59 to 8000.
Finally, the overall read depth (shown in Figure 4B) on the second chromosome relative to that on the X plus the third chromosomes indicates an excess of reads mapping to the second chromosome, although clearly not twofold.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com