Sentence examples for excess of staining from inspiring English sources

Briefly, cells were washed with PBS, fixed with paraformaldehyde for 30 min, incubated with 1% Alizarin red for 30 min at room temperature, and washed with PBS to remove the excess of staining.

After draining off the excess of staining solution by means of a filter paper, the specimen was transferred for examination in a transmission electron microscope (Tecnai Spirit 120 kV, Hong Kong).

With this method of subtractive normalization, a total of 11/48 (23%) tumors had mitochondrial content staining in excess of MDR1 staining.

The excess of stain was removed, and the plates were rinsed with deionized water and then dried.

Plates were then rinsed once with 85% propylene glycol solution and twice with distilled water to remove excess of stain.

Then, cells were stained using a crystal violet 1% (w/v) solution, incubated for 15 minutes and finally washed with sterile PBS to eliminate the excess of stain.

For relative quantitative measurements of TAGs accumulation, bottles were washed with PBS to remove excess of stain solution, dried, the colour was dissolved in 100% isopropanol and the absorbance was measured spectrophotometrically in a Victor 3 microplate reader PerkinElmer (Wellesley, MA, USA) at 500 nm.

Moreover, 8/11 (72.7%) tumors that exhibited mitochondrial staining in excess of MDR1 staining also demonstrated 99mTc-sestamibi uptake ratios greater than 0.6.

Conversely, 36/37 (97.3%) of tumors that failed to exhibit mitochondrial staining in excess of MDR1 staining also did not demonstrate increased 99mTc-sestamibi uptake ratios of greater than 0.6.

Similarly, the 99mTc-sestamibi uptake ratio was significantly elevated in cases in which mitochondrial staining was in excess of MDR1 staining (0.79 [95% CI 0.53 1.02] vs 0.27 [95% CI 0.23 0.32], respectively; Wilcoxon test p < 0.001).

Also, samples (tissue sections or isolated cells) should be incubated in the presence of preimmune and/or immune IgG preabsorbed with an excess of purified enzyme to establish negative staining (negative controls).