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Excess of primary antibody was removed by rinsing with TBS-T, followed by 1 h incubation with the species-appropriate horseradish peroxidase-conjugated secondary antibody (Anti-mouse Ig diluted 1∶40000 in the blocking solution) at room temperature.
In all of the above immunochemical experiments, the excess of primary and secondary antibodies were removed by intensive washings with 0.1% Triton X-100/PBS.
Pollutants in excess of primary drinking water standards were (in order of most frequently detected): iron, arsenic, selenium, gross alpha, radium and uranium.
22 A consistent excess of primary smoking-related cancers such as those of the oral cavity, pharynx, larynx, lung, and bladder have been reported in patients previously diagnosed with similar malignancies.
Among the unique challenges to meeting consumer expectations of a sustainable, sufficient, and secure food system are the resistance to production agriculture systems in certain communities, the not in my back yard (NIMBY) syndrome, and food waste that contributes to an insufficient supply of food for some populations in spite of an excess of primary food production.
Every excess of primary antibody was then removed twice using wash solution for 10' and immune-reactive bands were subsequently incubated with goat anti-mouse and anti-rabbit IgG-HRP secondary antibodies (1 2,000) in blocking solution for 1 h at room temperature.
Similar(53)
An excess of second primary esophageal cancer following first primary cancers of the oral and pharyngeal carcinoma was confirmed.
We found some evidence of significant excess use of primary care services in the 12 months following GBS diagnosis among patients with preceding ILI, with a median of six excess consultations compared with the 12 month period preceding GBS diangosis.
It also argues that the future of children at Greatstone Primary School, which has in excess of 360 primary age and 50 nursery age children, should not be jeopardised by the extension of the runway and "the potential increase in large passenger aircraft".
City Comptroller Alan G. Hevesi, one of the Democrats running for mayor, asked the city's Campaign Finance Board in January to consider lifting the spending cap on the Democrats if Mr. Bloomberg entered the race and spent far in excess of the primary limit.
Overall a slight excess of second primary cancers (relative risk 1.35) was observed.
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