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The increased confidence interval observed in Fig. 2 indicates that the application of the heterozygote-excess method to such a sample reduces the reliability, although the method still gives an unbiased estimate.

New developments suggest that they can be cancelled out with recent applications of a 17O excess method for the evaluation of aqueous productivity.

The heterozygosity excess method, on the other hand, was better at detecting bottlenecks when they were recent, less severe and when pre-bottleneck population sizes were small.

However, Nomura only considered populations with tiny Ne (<15) and only compared his new method to the heterozygote excess method, which was also the only single-sample estimator that Wang (2009) compared his new method to with simulated data.

The heterozygote excess method compares expected Hardy-Weinberg values to the increases in the observed number of heterozygotes (Pudovkin et al. 1996).

First, we used the heterozygosity excess method (Luikart et al. 1998) as implemented in the program Bottleneck v1.2.02 (Piry et al. 1999) to test for recent reductions in population size.

The core was prepared from the thioxanthone (TX) and the polyamidoamine (PAMAM) by a divergent synthesis procedure using the reagent excess method starting from ethylenediamine (EDA).

This method was preferred over the 'heterozygote excess method' (Pudovkin et al. 1996) because the later was less effective for converging.

Bottleneck tests based on the heterozygosity excess method did not indicate severe preceding bottlenecks in any of the temporal groups or in the modern-day reference sample.

A signal of recent population bottleneck was detected in only one population (L1; P = 0.03) under the TPM using the heterozygosity excess method.

Signatures of genetic bottlenecks were detected in all 8 populations after 2003 using the M-ratio method, but in no populations when using a heterozygosity excess method, indicating differential sensitivity of bottleneck detection methods.