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The reaction was stopped using 10 mM DTE. Excess label was removed with 60% ammonium sulphate.
Protein was precipitated to remove excess label, and resolved on 6% (for myosin heavy chain) or 12% gels (for myosin light chains).
After incubation, the biofilm was washed with Brock media (pH 5) to remove excess label and images were taken by CSLM.
Excess label was removed by dialysis.
Excess label was removed by dialysis into the above buffer.
Excess label was then washed away in HNE.
Similar(40)
With their permission he took their excess labels home, hoarded them and sorted them.
Controls were performed with no extract, excess unlabelled consensus probe, and excess labeled mutant probe to assess the specificity of the assay.
The cells were pelleted and washed with PBS to remove excess labeling reagent.
Labeling reagents are usually present in large excess, and most of the analytical techniques require a sample workup to remove excess labeling reagents.
Although under these excess labeling conditions we often observe minor peaks related to non-DHAA labeling, these species never convoluted spectral interpretation.
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