Sentence examples for excess gdp from inspiring English sources

We have measured three aspects of FtsZ filament dynamics at steady state: rates of GTP hydrolysis, subunit exchange between protofilaments, and disassembly induced by dilution or excess GDP.

To assess if the interaction of SYT isoforms with actin and MHCs was dependent on GTPase activity, we incubated lysates with nonhydrolyzable GTP-γS or excess GDP to increase or decrease GTPase activity, respectively, and then immunoprecipitated with pSYT antibody.

Binding kinetics were measured under pseudo-first-order conditions with excess GDP over 5-ATR ParM. 5-ATR ParM

To test the stability of the GDP-PhuZ lattice, excess GDP was spiked into wild type or D190A-PhuZ polymerization reactions having 100 µM GTP.

We preparatively loaded Rab8A with the fluorescent GDP analogue mantGDP and monitored the Rabin8-catalysed time-dependent displacement of mantGDP in the presence of excess GDP as judged by the decrease in mant fluorescence (Fig 9B).

The buffer also contained 20 MBq/0.05 cm [S]GTP γS (0.05 nM) and increasing concentrations (10−10 10−5 M) of N/OFQ 1 17 in the presence of excess GDP (30  μM) in a final volume of 1 mL.

The Irish Fiscal Advisory Council, a watchdog, has suggested a hybrid measure in which 40% of the excess of GDP over GNP is added to GNP.

Finally, we found that an excess of GDP caused disassembly of protofilaments with a half-time of 5 s.

The reaction was started by adding an excess of GDP and GEF and the fluorescence signal was measured over time (excitation wavelength is 366 nm and emission 450 nm).

We also repeated the same exchange reaction with hydrolyzable GTP (again in 10-fold excess to GDP) but did not detect any GTP bound to human LIC.

The exchange of mGDP in the presence of excess unlabeled GDP can thus be measured in real time as fluorescence signal decay.