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The corona is formed by the excess fragments of poly(sodium methacrylate) blocks not involved in complexation with poly N-ethyl-4-vinylpyridinium bromide).
In non-cycling (G0-phase) lymphocytes the induction and the repair of excess fragments was measured by means of the premature chromosome condensation (PCC) technique and the distribution of breaks among cells was analysed.
There was also a tendency of inverse correlation between DNA-PK activity and excess fragments.
Chromosome breaks and gaps not accompanying a dicentric chromosome were defined as excess fragments in this analysis.
In all, 200 metaphase cells from each individual were analysed, and the numbers of excess fragments were counted.
There was a tendency of inverse correlation between DNA-PK activity and excess fragments although this correlation was not statistically significant.
Similar(51)
The frequency of excess fragment increased as DNA-PK activity decreased in PBLs.
In this analysis of spontaneous chromosomal aberrations in PBLs at least one excess fragment was exhibited by each of the 30 patients.
Fig. 31 Intermediate steps in clearing excess shell fragments.
To remove excess nucleotide fragments EXO and SAP (Fermentas) were mixed in proportions 1∶4 and subsequently 5.5 µl EXOSAP added to all PCR-products.
In the presence of excess AFF4 fragments 32 67, 2 73, or 2 98, 50% of TAR was shifted by 1.1 nM Tat-AFF4-P-TEFb complex.
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