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Scrape the excess compound with a sharp stick, like a pointed dowel.
Treated platelets were centrifuged at 1400×g for 10 min at room temperature to remove excess compound and resuspended with 0.05 µg.mL−1 PGin in modified Tyrode's-HEPES buffer.
After 3 h, the medium containing photosensitiser was removed, the cells were washed with PBS to remove excess compound and fresh, equilibrated medium was added to each well.
The treated beads were centrifuged and washed three times with cold washing solution (4% DMSO in PBS) to remove excess compound.
Saturated solutions of 1 and 2 were prepared by stirring excess compound in MeNO2 overnight at 35 °C before allowing the solution to cool and residual solid to settle.
Wipe away excess compound with your drywall knife.
Similar(50)
Melodramatic excess compounds the muddle.
By now, however, almost a decade of excess, compounded by matrimonial discord and fatherhood, was beginning to take its toll on Shepard, and, after failing to turn up for the opening night of Cowboy Mouth, he fled with his family to London: "I was into a lot of drugs," he told Time Out shortly after his arrival.
With the combustion/reduction furnaces and liquid nitrogen trap in the GC/C/IRMS system, the gas introduced to the IRMS ion source was much cleaner without any excess compounds but N2.
This could be attributed to lengthy storage of on-farm formulated concentrates by peri-urban farmers because of animal feed shortages observed in the dairy system due to lack of grazing fields thus forcing them to formulate excess compounded concentrates.
To remove the unbound excess compounds from the membrane fraction, we washed the membrane fraction twice with 100 µl buffer A (30 mM HEPES-KOH, pH 7.4, 100 mM potassium acetate, and 2 mM magnesium acetate).
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