The one exceptional transcript (Spo11-1) wascoreded as borderline up-regulated (1.5-fold criterion, log2 = 0.6) in am1-489 mutant versus fertile at 1.5 mm on the array but was not significantly changed based on qRT results.
In FlyBase, exceptional genes and transcripts are flagged with Sequence Ontology terms and/or standardized comments.
Based on the diverse characteristics examined above, we searched for transcripts with exceptional properties.
Under normal circumstances, splicing occurs only between the 5' and 3' ends of the intron on the same transcript, although exceptional trans-splicing is also observed in some organisms [ 2].
We summarize our current catalog of such exceptional gene models and events, including polycistronic transcripts, noncanonical splices, trans-spliced transcripts, noncanonical translation starts, stop-codon readthroughs, multiphasic coding exons, and ribosomal frameshifting.
The identification of 8% of the transcripts with antimicrobial polypeptides is exceptional.
The affected transcripts are not flagged; an exceptional translation flag is not necessary.
However, we do not ignore the exceptional (and often inconvenient) cases that are well supported, including polycistronic transcripts, noncanonical splices, and trans-spliced transcripts (see Crosby et al. 2015, which is the companion to this article).
Exceptional cases are also flagged in GenBank RefSeq transcript and protein entries (Table S2).
The city will have to submit a statement explaining Ms. Black's "exceptional qualifications," along with a formal résumé and her academic transcripts.
