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For the subsequent experiment, the same previous pattern was followed except using a new angle.
The IgA quantitative ELISA was performed in a similar manner, except using a double detection system of biotinylated anti-human IgA antibody (Southern Biotech, USA) followed by streptavidin-HRP conjugate (Dako, Denmark).
Transfections of miRNAs in the heterozygous conditions were performed as above except using a ratio of 2∶1 of target to vector in order to compensate for saturation of the polymerase II transcription machinery.
Each thread executes the same loop, except using a unique random number sequence.
The spectral methods were as above except using a shorter length data segment (NFFT = 180).
Neurons expressing GCaMP5K-PSD95 via lentiviral infection were imaged as indicated above except using a 100× objective.
Similar(46)
The radioligand [18 F]AFM (2) was synthesized by the reported method (Scheme 1) [27] except using an automated synthesizer as previously described for the synthesis of 4-[18 F]-ADAM [25].
For cloning into viral vectors, the same ligation procedure was repeated except using an XhoI/ XbaI-linearized pLNCX2 retroviral vector (BD).
The first and second strand cDNA synthesis was performed using the Superscript II system (Invitrogen, Dublin, Ireland) according to the manufacturer's instructions except using an oligo-dT primer containing a T7 RNA polymerase promoter site.
Do exactly what you did with the first color of glitter, except use a new colour that will nicely contrast with the first.
Except for using a charge card, it wasn't.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com