Sentence examples for except the template from inspiring English sources

PCR reaction conditions were the same with the 1st nested PCR except the template was changed into 0.1 μL product of the former nested PCR.

A negative control containing all the components of the PCR except the template was included in each PCR reaction.

A culture PCR is similar to colony PCR, except the template is not a single colony, but part of the induced culture, likely containing a mixture of inverted genotypes.

Negative control reactions were included in each run and contained all reaction components except the template.

A reaction mixture containing all of the ingredients except the template was made.

The sample containing all reagents except the template DNA was treated as the negative control.

For 3′ RLM-RACE, all the reverse transcriptase steps were the same as 5′ RLM-RACE, except for the template where 1 μg of total RNA was used.

The model-building process of this bulk structure was similar to that of the particles, except that the template lattice was shaped in a cubic cell with three-dimensional periodic boundary conditions.

The bridge connection method involves essentially the same procedure, except that the template fragment is translated so that atom N is superimposed on top of a bond projecting from the subject atom with the angle θ1 and an extent equal to the default bond length.

The original concentration of the gel-extracted amplicons were restored by 10 PCR cycles with the same Ta and reaction mix as used for the previous PCR, except for the template, which was 2 µl gel-extract in this case.

The procedure for real-time PCR analysis of DNA was identical to the one for RNA analysis except that the template nucleic acid was 5 ng DNA per reaction and the reverse transcription step was omitted.