Sentence examples for except the secondary from inspiring English sources

Immunocytochemistry was performed using antiserum against HSV glycoprotein D (Novagen, 1∶10,000), as described [49], except the secondary antibody was a FITC-conjugated donkey antiserum against mouse IgG (Molecular Probes, 1∶1,000).

Anti-PCNA FITC-labeled antibody (BD-Biosciences-Pharmingen,) (1∶100) staining was performed by the same procedure done in pH3 antibody staining as described above, except the secondary antibody.

There is no difference by education (except the secondary school group in the non-slum areas) and work status (except the retired group in the slum areas).

No more tickets are available, except through the secondary market.

CD11b immunohistochemistry (rat anti-mouse, 1∶500; 3 h, Serotec) was performed similarly to that for GFAP staining except that the secondary antibody was goat anti-rat (Vector) diluted 1∶1000.

The microscopy experiments in Figure 8 and 9 were performed as above except that the secondary antibodies goat anti-rabbit AlexaFluor 555 and goat anti-mouse AlexaFluor 488 were used to detect USP7 and PML, respectively.

Immunostaining was performed as described [71] except that the secondary antibodies Alexa Fluor 488 goat anti-mouse IgG or an Alexa Fluor 594 goat anti-rabbit IgG (Invitrogen) were used.

In fact, expression of miR-429, miR-200b and miR-200c was elevated in the highly metastatic 4T1 cells but absent from 4TO7 cells, which can perform all of the steps leading up to the establishment of lung metastases except establishing the secondary tumor.

For Figure 9A, the staining was performed as just described, except that the secondary antibody to detect Vpu was anti-rabbit conjugated to FITC and the secondary antibody to detect AP1 was anti-mouse conjugated to rhodamine red-X.

Note that results for the Time layer are obtained independently from any information on Nesting Structure (except for the secondary inferences of bounds on the ages of overlapping elements).

Membranes were then incubated in mouse monoclonal anti-GAPDH antisera (1 40,000) and processed as described above except that the secondary antisera were goat antimouse horseradish peroxidase antisera diluted 1 10,000 (Jackson ImmunoResearch, Baltimore Pike, West Grove, PA).