Sentence examples for except that the secondary from inspiring English sources

The microscopy experiments in Figure 8 and 9 were performed as above except that the secondary antibodies goat anti-rabbit AlexaFluor 555 and goat anti-mouse AlexaFluor 488 were used to detect USP7 and PML, respectively.

Immunostaining was performed as described [71] except that the secondary antibodies Alexa Fluor 488 goat anti-mouse IgG or an Alexa Fluor 594 goat anti-rabbit IgG (Invitrogen) were used.

CD11b immunohistochemistry (rat anti-mouse, 1∶500; 3 h, Serotec) was performed similarly to that for GFAP staining except that the secondary antibody was goat anti-rat (Vector) diluted 1∶1000.

Membranes were then incubated in mouse monoclonal anti-GAPDH antisera (1 40,000) and processed as described above except that the secondary antisera were goat antimouse horseradish peroxidase antisera diluted 1 10,000 (Jackson ImmunoResearch, Baltimore Pike, West Grove, PA).

They were then probed immunologically as previously described [ 34], except that the secondary antibody was goat anti-rabbit IgG (diluted 1 7500 in TBS-T and 1% BSA) conjugated to horseradish peroxidase (HRP).

For Figure 9A, the staining was performed as just described, except that the secondary antibody to detect Vpu was anti-rabbit conjugated to FITC and the secondary antibody to detect AP1 was anti-mouse conjugated to rhodamine red-X.

From the societal perspective, the cost-utility results are similar to the results from the health care perspective except that for the secondary CVR prevention samples the uncertainty about the incremental costs became even larger.

Williams would have moved into the starting job then except that injuries in the secondary last year made him too valuable as a nickel defender.

Immunostaining of polytene chromosomes was carried out as described [37] except that Alexa Fluor secondary antibodies and the following primary antibodies where used: α-dCTCF (1∶100), α-JIL-1 (1∶100), α-Mod mdg4 2.2 (1∶200), α-Mod mdg4 2.20), α-CP1∶2001∶300), α-Supo II (1∶200).

But incredibly, the newspaper also said that Van Brocklin might have had even more yards except that the Yanks' secondary covered well.

That's the secondary issue.