Sentence examples for except that the medium from inspiring English sources
The S. aureus biofilm samples were prepared in the same way except that the medium was LB plus 10 µg/mL chloramphenicol and the incubation time was reduced to 24 h due to a higher growth rate of S. aureus.
The other aliquot was also divided, transferred to six flasks and similarly treated, except that the medium was supplemented with N, to provide controls.
Immortalized Nrf1−/− mouse embryonic fibroblasts (MEFs) (Radhakrishnan et al., 2010) were grown as above except that the medium was additionally supplemented with β-mercaptoethanol and non-essential amino acids (Invitrogen).
The lines in the coevolution treatment were subjected to selection by coevolving N. whitei, while the same lines in the control treatment were always kept and handled in identical ways, except that the medium was parasite-free and the hosts thus uninfected.
Each bacterial strain was competed against a metC::Tn 10 derivative, called BW3454 (Table 4), of the BW2952 ancestral strain [ 29], under the same conditions used during the chemostat evolution experiment, except that the medium was supplemented with 4 μg ml-1 methionine.
The tubes were sealed with parafilm and submerged in a water bath at 43°C for an additional 5 to 40 min. Spermatocytes were heat shocked also at 38°C in a similar way except that the medium was pre-heated to 53°C.
To induce tracheids, callus cells were suspended in EDM liquid medium and transferred to induction media following the same procedure as described by Möller et al.[ 56], except that the induction medium was EDM medium without hormones instead of EDM medium supplemented with activated charcoal.
Treatment of cells was similar for the dose response experiments, except that here the medium was exchanged daily (supplemented with the indicated Dox-concentrations) as a precautionary measure to account for the possible degradation of Dox at very low concentrations.
From these "crude" cultures, a single cell was picked to establish a clonal strain by the same micromanipulation procedure used in crude isolation except that MA medium was used for clonal cultures.
A similar sequential protocol was employed in the suspension cell lines, except that medium was not aspirated from the cells after the 24-h pre-treatment.
