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For plasmid transfection, the procedure was the same except that subsequent experiments were carried out from 24 hours post-transfection.
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All eight mutations at the sites shared between Wichman et al. (2005), and this study was the same except that a subsequent mutation at 1301 (to a T) did not exceed the threshold in this study and the two mutations that were coeval at site 1639 were sequential in Wichman et al. (2005).
Because average and single linkage clustering algorithms are considered to be less conservative and more dependent on sampling intensity [47], we used the complete linkage algorithm with default parameters, except that we collapsed subsequent gaps to avoid overestimating distances from the rapidly diverging V6 region.
Briefly, following treatment, 1,000 viable cells (as determined by trypan blue staining) were plated into six-well plates in complete medium without zVAD-fmk, CHX, TRAIL or TNF, cultured for 7 days at 37°C and stained with crystal violet as described above under "viability assays", except that all steps subsequent to washing with tap water were omitted.
Nothing is known of his role in the subsequent events until 540, except that it was probably at about this time that he was raised to the rank of patricius.
At S1 and S7 the compartments had been cleaned and were ready for the subsequent batch of birds except that the bedding was not in place.
Young and his crew undertook each aspect of that subsequent mission except for an actual moon landing.
Except that I do.
Except that it has.
Except that they do.
Except that she didn't.
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CEO of Professional Science Editing for Scientists @ prosciediting.com