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For each locus (except loci 8, 10, and 61), an 18-bp tail of M13 was added on the forward primer (Additional file 2).
All loci showed significant linkage disequilibrium (except loci ParJ_31 in the Lauris population and ParJ_37 in the Lauris and Aix-en-Provence populations).
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All loci, except locus 15 in A. radiatus, amplified successfully within the expected allele size in all species.
Analysis of loci over all populations with LOSITAN suggests that loci are putatively neutral except locus STN196, which may be under positive selection, and STN19, which may be under balancing selection.
Control for confounding by population stratification eliminates these associations, except at loci that are linked to candidate genes for the trait.
The microsatellites generally exhibited high levels of heterozygosity and, except for loci Cma10EPA, Cma11EPA and SP251, observed heterozygosity was not-significantly higher than expected heterozygosity.
FIS results for island wolves were positive for all except two loci, with a mean value of 0.211.
These sites are located in non-coding regions (except for loci 61 in ycf1) and can thus be considered as mostly neutral.
In general, these interacting genomic areas showed the highest d × d interactions in comparison to other single epistatic effects, except the loci on SSC2 and SSC7, where the d × a interaction was the most prevalent.
The latter falls almost exactly on the MLE orientation of the X marker (Btk) and within the two unit support bounds for the orientation of every other locus except Np and the Y chromosome, while the support intervals for all loci except the Y overlap with that of the consensus.
The result showed that WY17 1-1 WY17 1-1almost same genetic background as 93–11, except thadlocus of Bph6 and Bph9 inthegresame from genetic background of Swasnalata and Pokkali (Fig. 1).
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CEO of Professional Science Editing for Scientists @ prosciediting.com