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Cytotoxicity and cell death were always determined 18 h after SNP incubation (except for time course assay), using the assays described below.
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For time course stimulations, cells were incubated in starvation media (supplemented RPMI media except with 1% FBS overnight at 37°C).
All cells (except for the time course study) were grown up to the stationary phase for 48 h at 23 °C or 24 h at 28 °C at an agitation rate of 200 rpm.
Fiber length (cm) was measured at Lo. Diaphragm contractility was assessed in all experiments, except for the time course studies.
Except for time, of course, and the depths of the ocean.
All samples and standards were run in triplicate, except for the azaspiracid time course which was run in duplicate.
Except for the time-course experiments which used duplicate dishes.
Time course for resolution.
Time course for MUC5AC secretion.
All these operons, except for CAC2705, were highly overexpressed during the time course of all the tested butanol and butyrate stresses in this study, which is consistent with previous reports.
The time course of CREB phosphorylation resembled the time course of cAMP production, except for EdTx at late time points.
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