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Fiber length (cm) was measured at Lo. Diaphragm contractility was assessed in all experiments, except for the time course studies.
HRP-conjugated (Jackson ImmunoResearch, West Grove, PA) secondary antibodies were used for protein detection in all experiments except for the time course and proteasome inhibitor studies.
All cells (except for the time course study) were grown up to the stationary phase for 48 h at 23 °C or 24 h at 28 °C at an agitation rate of 200 rpm.
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Except for the time-course experiments which used duplicate dishes.
All these operons, except for CAC2705, were highly overexpressed during the time course of all the tested butanol and butyrate stresses in this study, which is consistent with previous reports.
All model parameters are shown in Table 2 and were derived from the experimental values in Table 1 or obtained from the literature, except for the intrinsic extrusion rate γ0 and the time course of the calcium current σ.
The time course of CREB phosphorylation resembled the time course of cAMP production, except for EdTx at late time points.
Genes in cluster 3 steadily decreased during the time course of the experiment, except for VEGF, which increased at 2 hrs then was gradually down-regulated.
The time course of the NLI was in parallel to that of the ERK phosphorylation except for a slight delay seen at the onset.
All samples and standards were run in triplicate, except for the azaspiracid time course which was run in duplicate.
For the time-course experiment, cells were treated similarly except that cells were treated with 20 n m siRNA initially and then every 48 h throughout the time-course.
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