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Dwarf males are much smaller than the host barnacle in which they live and are degenerate, except for the testes.
All extracted organs from all animals were fixed in a 10% neutral formaldehyde solution, except for the testes, which were fixed in Bouin's solution.
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The testes then produce testosterone, which issues a second set of developmental instructions: for a scrotal sac to develop and for the testes to descend into it, for a penis to grow, and so on.
Many sex-biased genes were restricted to the gonads (3,890 female-biased and 3,298 male-biased) (Welch's two-sample t-test; t2 5 FDR < 0.05; fig. 1 B) and gonadectomized abdomen (2,447 female-biased and 3,181 male-biased) (Welch's two-sample t-test; t3 6 FDR < 0.05; fig. 1 C), which still contained numerous reproductive organs except for the accessory glands, testes, and ovaries.
Digestion by McrBC was quality controlled by qPCR analysis of the promoter of the TH2B gene, which is densely methylated in all tissues except the testes [47].
Tissues were fixed in neutral buffered 4% formalin, except the testes, which were fixed in Bouin's solution.
Expression of TUBB4a in other body tissues was very low except for moderate expression in the testes (Fig 5).
Moreover, all tissue-specific genes, except for those specifically expressed on the testes or the ovaries, are randomly distributed on the chromosomes.
Physical examination of the patient, including the testes, was normal except for a systolic heart murmur (preexisting and known to the patient), and clinical signs of left pleural effusion.
Except for the freakish exceptions.
The ex vivo biodistribution follows mainly the mRNA pattern of A3 described previously [ 3], except for the lack of pronounced tracer uptake in rat testes [ 11].
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