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Except for the templates in the 16 groups of Fig. 18, the majority has a very low number of representing reactions.
Single-stranded DNA (ssDNA) was generated by using the same conditions in multiplex PCR except for the templates that were 10 μl of the multiplex RT-PCR product.
Single-stranded DNA (ssDNA) was generated by using the same conditions in the multiplex PCR step except for the templates that were 2 μl of the multiplex RT-PCR product.
Similar(57)
For 3′ RLM-RACE, all the reverse transcriptase steps were the same as 5′ RLM-RACE, except for the template where 1 μg of total RNA was used.
The original concentration of the gel-extracted amplicons were restored by 10 PCR cycles with the same Ta and reaction mix as used for the previous PCR, except for the template, which was 2 µl gel-extract in this case.
First, we conducted a RNA synthesis reaction with T7 RNA polymerase under the same NTP concentration and ratio between radioactive and nonradioactive GTP (1/1600) as the IAV reaction except for the template sequence.
To reduce variability among replicates, PCR premixes, which contain all reagents except for the template, were prepared and aliquotted into 0.2 ml thin well plates.
Using four DNA duplexes with essentially the same sequence except for the templating bases, we measured the rate of incorporation of each correctly paired dNTP by WT Pol I KF) under single-turnover conditions.
As for the origin of polymerases, I do not know of any ribozyme counterpart or RNA constituent for any known polymerase, except for the RNA template of telomerase.
Similar trends of results were observed with four DNA lesion templates, except for the case with the template N-EtG and the R96G variant.
Cut out the circle for the template.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com