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In the process, the slides were all rinsed three times in phosphate-buffered saline (PBS) (pH 7.2) before each step, except for the procedure of serum blocking to incubation with the primary antibody.
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The non-stressed control mice were submitted to the same handling at the same time except for the immobilization procedure.
Nevertheless, MCMCglmm was the winner this time, but we considered all computation times as acceptable, except for the SAS MCMC procedure which took 37 hours for the binary model.
It is important to point out that the LNO results vary for each run because the partition of the data set is changing in a random manner (except for the LOO procedure).
Paired analyses revealed no significant differences between parental and subject responses except for the pre-procedure and immediate post-procedure periods when parents rated their children as more distressed than the children themselves.
Except for the photosensitiser, this procedure followed the protocol optimised in our previous studies (Korbelik and Sun, 2006).
Moreover, except for the statistical validation procedure, all the other algorithms are standard and have been used in published microarray papers.
In all experiments except for the egg collecting procedure, the nematode B. okinawaensis was propagated on the budding yeast Saccharomyces cerevisiae, strain W303-1A, on 1/10 malt extract agar medium (MEA) (Difco) with 4% agar containing 100 µg/mL chloramphenicol in a 60-mm Petri dish (hereafter called 1/10 MEA-chloramphenicol plate).
For comparison, a control catalyst resembling the original Pd/HDPE catalysts [5] was prepared following the same mentioned procedure, except for the supercritical foaming process.
In meiotic conjugation cycle, only the micronucleus takes part as one of the similar biological processes which are evolutionarily conserved with multicellular organisms meiosis, except for the extra complicated procedures of several successive mitotic cycles in conjugation after meiotic segregation (Collins, 2012).
No identifying data was recorded except for the type of endoscopic procedure being performed (gastroscopy or colonoscopy) and which month of the 3-month endoscopy rotation the resident had attained at the time of the procedure.
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