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In prokaryotes, except for the expression system used by Tran et al. (2010), which allowed the production of the Bacillus sp. MD2 phytase at 327 U ml-1 by fed-batch cultivation, the majority of earlier attempts with expression of Bacillus phytases in Escherichia coli have resulted in production of inclusion bodies which entails additional steps for recovery of the active enzymes (Rao et al. 2008).
The NC-TDI assay was performed in a similar way as a standard transactivation assay, except for the expression vectors that were used.
Although significant problems still remain, many transgenic animals behave normally and do not show any differences from non-transgenic animals, except for the expression of heterologous protein [32], [41], [42].
Except for the expression of ACO2 and ETR2 in the leaves, the transcript levels of the ethylene responsive genes decreased compared to 24 h of exposure to Cd.
With AMX, except for the expression of ponC, there were no detectable differences in the expression of genes encoding penicillin binding proteins (pbp).
The data were analyzed mainly with the help of packages in R [ 35] except for the Expression Analysis Systematic Explorer (EASE) analysis [ 36], which was performed in MEV [ 37].
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Except for establishing the expression conditions for BVMOs that could not be expressed before, we also identified two new BVMO genes (BVMO8 and BVMO24).
Except for OsXYLP19, the expression levels of all examined genes were increased under GA treatment.
Except for PtSPL17, the expression levels of 27 PtSPLs in roots, stems, young leaves and mature leaves were obtained.
However, except for NtGPX, the expression of the other five antioxidant enzymes examined was reduced in unstressed transgenic seedlings compared with the wild-type plants.
Surprisingly, except for TcLec1, the expression of these genes is higher in M-SE samples compared to the less mature LT-SE.
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