All ligands of each dataset were clustered with LigandScout using default settings except for the cluster distance that was adjusted for each NR to obtain balanced clusters.
Figure 7 also shows that lowering the thresholds does not considerably affect the number of located runners, except for the cluster size S.
Thus, we performed a first step of ligand clustering using the LigandScout Ligand-set Clustering tool with default settings except for the cluster distance threshold that was manually adapted for each NRLiSt BDB dataset.
However, more importantly, we found that, except for the clustering coefficient, almost all average network properties of each module significantly (P<0.05) differ from the corresponding global average of the whole brain networks (Table 1 and Table S5).
Between the three conditions significant changes were observed for all functional annotation clusters, except for the cluster of acid phosphatases.
That is, except for the cluster in scaffold02229, no other clusters contained RGHs that were annotated in the same class.
All age clusters are statistically different from the younger volunteers except for the cluster of those aged 40 years to 49 years, for which the difference is only significant at 10%.
Except for the clustering of W. halotolerans and W. oryzae with W. koreensis, all other relationships were maintained, with W. paramesenteroides closely related to W. confusa and W. cibaria, whereas W. halotolerans is more distantly related.
For both front hind times and left right times, we only evaluated steps with step lengths of 2 or 4. Except for the cluster analysis (see below), data were analyzed using SPSS IBM Corporationn, Armonk, NY, USA) and all p values were calculated by comparing cerebellar mutant mice with their control littermates.
