The dbSNP and ALFRED numbers of each SNP from the ADH clusters are listed in Figure 2. All SNPs were genotyped by the TaqMan method [72] using commercial assays and reagents (http://products.appliedbiosystems.com) except for the assay for ADH1B Arg47His, which we designed [30].
Cells were fixed with 4% paraformaldehyde and subjected to the PLA, except for the assay that detected MYPT1 phosphorylation, in which 10% TCA was used as a fixative to preserve the phosphorylation.
The study was supported by the Health Research Council of New Zealand grant 05/131 (Early intervention in acute renal failure), except for the assays of cystatin C, which were supported independently by the Christchurch Kidney Research Group.
In addition, the range of potency values was consistent across the assays, except for the comet assay, which demonstrated higher variability.
A negative control and a positive control for the reaction were included in each assay, except for the SNP assay in which only a negative control was included, since the wild-type allele acted as a positive control for the reaction.
bAll serologic assays were in-house enzyme immunoassays (4 ), except for the Meridian IgM assay for M. pneumoniae (Meridian Bioscience, Inc., Cincinnati, OH) and enzyme-linked immunosorbent assay of the Centers for Disease Control and Prevention for SARS-CoV.
While in those with regular menstrual cycles, blood samples were obtained during the follicular phase of the cycle, except for samples utilized for the assay of progesterone that performed in the day 21 of the cycle (luteal phase).
The diagrams for the characterization of immobilized ScLac preparations were based on relative activities calculated by assigning the highest value in each dataset as 100% (except for the reusability assay).
The Y strain of T. cruzi was used in all experiments except for the metacyclogenesis assay, in which the Dm28c strain is the best characterized model for in vitro differentiation [18].
The PCR parameters for dog, guinea pig, cow, chicken, cat, mouse and pig DNA [33], [34] were: initial denaturation of 1 min at 95°C, followed by 30 cycles of 95°C for 30 s, annealing as shown in Table 1 for 30 s, and 30 s of extension at 72°C, except for the porcine assay where 1 minute to anneal and extend at 63°C was used.
All data were analyzed by Mann Whitney tests except for the ChIP assay in which ANOVA tests were used.
