Results for subjects aged 40 y and under were combined for subsequent analyses, except for ALT results in males.
Three days after seeding, the propagated spheroid bodies were collected and digested by StemPro Accutase (Thermo Fisher, Waltham, MA, USA) to single cell suspension for subsequent experiments except for 3D infection test, in which they were directly used.
Because mate choice was found to have less effect on the maintenance of cue-dependent cooperation when x = 0, we set x = 6 for subsequent simulations (except those in Figure 3C), i.e. we assumed that matings between individuals with non-identical recognition genotypes produce additional offspring, equivalent to being helped 6/ b times.
This trend continued, but with a declining magnitude of the odds ratios for subsequent age categories, except the age category 40 44 in which the likelihood of non-adherence was low and not different from that for the 45 49 age category (OR = 1.31, 95% CI 0.95-1.83).
Do this for subsequent layers.
Be prepared for subsequent attacks.
DeFeo's seven years of constant toil on the painting came to an end (except for some desultory subsequent touches) only when she was evicted from her apartment, on Fillmore Street, in November , 1965
Nixon biographer Herbert Parmet noted, "Except for Nixon's subsequent reputation, what happened in California's Twelfth would have been indistinguishable from campaigns across the country to elect the Eightieth Congress".
Except for SP-B content subsequent analyses of the obtained surfactant preparations showed no significant differences regarding their phospholipid and apo-protein profile.
For plasmid transfection, the procedure was the same except that subsequent experiments were carried out from 24 hours post-transfection.
