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The microsatellites generally exhibited high levels of heterozygosity and, except for loci Cma10EPA, Cma11EPA and SP251, observed heterozygosity was not-significantly higher than expected heterozygosity.
These sites are located in non-coding regions (except for loci 61 in ycf1) and can thus be considered as mostly neutral.
Except for loci on chr02, the proportions of AJ alleles of most markers in both the selected and unselected populations of parasites were generally high, possibly reflecting the predominance of AJ alleles in the backcross, the small proportion of parental AJ genotypes (drug-sensitive) and/or the existence of many AJ loci contributing small fitness effects in this experimental system.
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Crohnbach's alpha ranged from 0.56 to 0.86, except for locus of control (α = 0.02).
All loci were polymorphic, except for locus Mce-8, which was monomorphic in some of the samples.
It also did not reveal any hint for existing null alleles, except for locus C5 in Dp.
Crohnbach's alpha for internal consistency ranged from 0.56 to 0.87, except for locus of control (α = 0.02).
For all 21 loci, except for locus Lb_34, DNA bands were successfully amplified using the samples from all four populations of L. boninense.
All the predicted genome locations of these loci were autosomal except for locus CAM-11, which was predicted to be Z-linked.
We detected the presence of null alleles using MICRO-CHECKER (van Oosterhout et al., 2004) and had no indication of null alleles except for locus DH-1-118 DH-1-118 DH-1-118
This feature was verified using a VNTR analysis program [ 75], and most of identified loci were correctly predicted as VNTR, except for locus 3 in B. pertussis and B. bronchiseptica, and for locus 4 in B. bronchiseptica (data not shown).
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