Exact(2)
Despite the plan she held and the timer, the self-possessed 12-year-old Willow Parchment looked like a child left home alone, with nothing particular to do except experiment with her parents' stuff.
Two complementary (with 2-nt 3′ overhangs) RNA oligos (50 pM each) were labeled by incubating with 50 μCi of gamma-P-ATP, 20 U of Optikinase (GE Healthcare), and 1× Optikinase buffer in a 50-μl reaction volume at 37°C for 30 60 min. For all of the loading assays except experiment shown in Fig 7F, sR-3hit-G and sR-3hit-aG were used as RNA oligos.
Similar(58)
Experiments were repeated three times in 6 replicates, except experiments when two different miRNAs were co-transfected simultaneously which was done only once in six replicates.
For B. subtilis all the available microarray experiments in the KEGG Expression Database (www.genome.jp/kegg/expression) were incorporated except experiments that increased the number of false positives; 69 experiments in total.
All experiments were performed in serum-free media except experiments for IL-18 detection.
All treatments in this study were conducted in triplicate (except experiments with growing duckweed and azolla in wastewater, where five replicates were used for each treatment).
Mobile sparklets were captured at 33 Hz at 100% laser power while immobile sparklet intensity experiments (except experiments in Figure 1A and Figure 1 figure supplement 1) were recorded at 50 Hz at 20% laser power.
Ovalbumin antigen (200 μg/ml) or SIINFEKL peptide (1 nM) was added to the cultures, except experiments involving Lm-NT-OVA-RFP patasites, and incubated at 37C, 5% CO2 for 72 h.
The fluorescence measurement of the kinetics of Mg·formycin-A-5′-triphosphate (MgFTP) binding and displacement by MgATP were as described by Geeves et al., except experiments were performed at 30 °C.
The addition of nitrogen increased the total DMI and SCT intake in all experiments, except in experiment 1, where, TG had a substitution effect on SCT intake.
All experiments except the experiment presented in Fig S3 were performed using CB6F1 mice.
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