Sentence examples for except cells from inspiring English sources

Cells were incubated at 37 °C in 5% CO2 except cells cultured in Leibovitz's L-15 medium, which were grown without CO2.

For studies utilizing GSF cell lines, cells were treated in an identical manner except cells were not transfected with the reporter construct.

The quantification of ROS was performed as previously described [39] except cells were incubated in 1% serum at 37C for 16 hours prior to staining with 2.5 µM CM-H2-DCFDA (Molecular Probes-Invitrogen).

Expression and immunoprecipitation of EPHB2 variants for in vitro kinase assays was performed as above except cells were left unstimulated prior to cell lysis and immunoprecipitation, as previously described by Holland et al. [16] Gels were analyzed and quantified using a Storm phosphoimager (Molecular Dynamics Inc., Sunnyvale, California, USA).

Formation of the SDS-resistant PA63 7) pore species was detected by immunoblotting as previously described [24], except cells were incubated with 2.5×10−8M PA and 2×10−9M LF for 2 hours at 4°C, washed and shifted to 37°C for the indicated times.

Immune cells (granulocytes, lymphocytes) showed constantly high expression of Progranulin except cells of lymphoid follicles.

All photos by Christopher Burke except "Cell (Clothes)", which was shot by Attilio Maranzano.

The RH30 TBX3-1 cell line was transiently transfected with pEF TBX2 (TBX2) or pEF empty vector (EV) and assayed for proliferation as in (a) except cell counts were performed every 24 h.

Total cell lysate protein preparation, SDS-PAGE, and immuno-blotting: as described [80], [82], except Cell Signaling antibodies, where supplier's protocol was followed precisely.

A 5 kHz low-pass filter was used for all experiments except cell-attached patches in which 1 kHz filtering was utilized.

Except cell sorting, all FACS analysis was performed three times.