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For the synapsis-by-protein-dimerization model, as exemplified by Tn10 and Tn5, we make the same assumptions as detailed above, except that the free transposase is monomeric.
In order to determine if the two discovered Dlx genes were closely linked, genomic walking was performed by designing probes to the end sequences of the parental phage and screening the library as detailed above, except that hybridisation temperatures were raised to 42°C and post-hybridisation washes were increased to 65°C.
The D population was treated with NMU as detailed by Kerr and Sebastian, except that volumes were reduced by 1/10th [ 30].
The synthesis of the activated chromophore, 4-hydroxycinnamic acid S-thiophenyl ester, was conducted as detailed by Changenet-Barret et al., except that the product was not recrystallized.
All children except the AM group underwent the full battery of psychometric tests as detailed in Table 1.
Each operation was performed once for each repository, except for the repository registration, which was executed twice ("Insert 1st" and "Insert 2nd"), as detailed below.
As detailed in Table 5, 81 CD630 genes are absent, or highly divergent, from all other PCR-ribotypes (except the PCR-ribotype 012 reference strain).
For each protein (except SmMHX and CrNCX), only residues that were totally conserved in the whole group it represented were highlighted, either in gray or as detailed below.
