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Experiment 2 was identical to experiment 1, except as described below.
Except as described below (" Accurate mass-directed tandem mass spectrometry"), mass spectra were obtained using data-dependent acquisition.
Do not add water, as you did for an omelette, except as described below.
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The authors' contribution was as described below.
After drying the immunodectection was carried on as described below.
Assemblies were generated using SOAPdenovo (default parameters except −K as described below) using Illumina reads for both contig generation and scaffolding (rank = 1, pair_num_cutoff = 3, asm_flags = 3, map_len = 32) and Sanger reads for scaffolding only (rank = 2, pair_num_cutoff = 3, asm_flags = 2, map_len = 32).
Food and water were available ad libitum (except during torpor experiments, as described below).
When characteristics were not recorded comprehensively, we treated missing data either recorded as "unknown" or not ascertained as a separate value and included them in the analysis (except in some sensitivity models, as described below).
Where the method of admission indicated a previous outpatient attendance, the most recent outpatient activity in the 6 months before the admission was identified and the source of referral of this outpatient attendance examined as described below, except that if there was no outpatient activity within 6 months before the inpatient admission, the route was classified as Inpatient Elective.
After splitting the maps at these low-coverage regions, we used OMBlast to align the split fragments with a minimum of 10 signals to reference maps and to detect large insertions, deletions, and inversions using partial-alignment-based strategy as described below, except that the minimum number of fragments supporting the variant call was set to one.
Construction of the DNA library for Illumina sequencing was as described below except for the size of the DNA fragments used: ∼250 bp fragments, corresponding to ∼150 bp DNA fragments isolated by ChAP without adapter sequences, were selected for PCR enrichment.
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