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Smad7 is expressed at exceedingly low levels in normal keratinocytes (He et al., 2001), but is often overexpressed under pathological conditions (He et al., 2002; Han et al., 2011).
The use of the FRET biosensor in a combination with other analytical methods determined the presence of exceedingly low levels of active furin on the cancer cell surface.
Surprisingly, despite high amounts of the loaded protein material, which corresponded to 12×106cells/lane, exceedingly low levels of furin were detected in the biotin-labeled MCF-7 furWT MCF-7 furWTs (Fig. 7).
Using the biosensor that was cell-impermeable because of its size and also by other relevant methods, we determined that exceedingly low levels, if any, of cell-surface furin are present in the intact cells and in the cells with the enforced furin overexpression.
Thus, targeting those patients with exceedingly low levels of the miRNA and delivery to the appropriate cell type or tissue are important aspects of effective miRNA mimicry.
This is probably due to the fact that LNX1 and LNX2 proteins are expressed at exceedingly low levels in cell lines and in vivo[ 18, 20].
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"[That] fails because of our exceedingly low exposure levels".
But by 1888, a French company was making bread for diabetics with soybean flour, thanks to the bean's exceedingly low carbohydrate levels.
12 In contrast, after infecting keratinocytes, human papillomaviruses have exceedingly low expression levels and thus avoid detection until they begin replicating inside cells that are about to be shed.
The suppression of androgens by androgen deprivation therapy (ADT) alone or together with androgen receptor (AR) antagonists initially induces tumour regression and a period of cancer control, accompanied by nondetectable or exceedingly low PSA levels [ 5].
Our LSPR-based quantification showed that miR-10b is present at high concentrations (∼210 fM) in exosomes isolated from the plasma of PDAC patients, whereas the supernatants post-centrifugation (Sup-1, ∼10 50 fM; and Sup-2, 70 300 aM) had exceedingly low miR-10b levels.
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