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Example of scoring for quantification of EphA4, pTau and Aβ.
The detailed example of scoring matrix is provided in Additional file 7.
We devised a scoring system to visually assess the quality of the peaks, for example, resolution of peaks, height of intensity and quality of baseline (See additional file 4: Optimising experiments, for an example of scoring system).
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Examples of scoring according to staining intensity and the percentage of stained cells are shown in Figures 1 and 2. PSA progression-free survival and overall survival were the main end-points of the present analysis.
Twenty-four hours post-irradiation (the time required to clear TD lesions), the UVB-irradiated areas were biopsied and the basal layer keratinocytes were assayed for co-expression of proliferation (Ki67) markers and UVB-induced DNA damage (TD; see examples of scored keratinocytes in Supplemental Figure 4).
An example of score calculation is available in supplementary Figure S2.
Detailed guidelines with examples of scoring are also given in the AMIPB.
Throughout the scoring, examples of cores scored as 1, 2 and 3 were on hand for comparison as an "internal standard".
Yet, there are many examples of scores losing accuracy in particular patient populations, including patients with human immunodeficiciency virus [ 26], cardiogenic pulmonary edema [ 27], and trauma patients [ 28]; even APACHE-IV significantly under- or over-predicted mortality in 13% of medical conditions during its validation [ 10].
Table II provides an example of the scoring process; the factors taken into consideration in the complete scoring process are set out in the final pages of the dictionary [ 29].
Fig. 1 Example of preference scoring.
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