Computational analysis of genome-scale metabolic reconstructions has, for example, been used for biochemical [3, 4], biomedical [5 7], and bioengineering [8, 9] purposes.
For example, in biochemical assays for disease diagnosis, a titer of antibodies directed against Epstein-Barr virus or Lyme disease is estimated from the blood.
For example, the biochemical condition (Calcium concentration or pH) for enzyme activation could be different according to each PADs.
For example, biochemical studies suggest that SERCA is located throughout the SR [ 11].
For example, biochemical assays routinely quantify reduced and oxidized glutathione, or quantification of protein thiols and protein disulfides.
For example, biochemical studies of these domains uses detergent extraction and sucrose gradients to isolate the low-density, detergent-insoluble fraction enriched in cholesterol and glycosphingolipids [89].
For example, biochemical interaction between TSLP and IL-7 receptor alpha subunit and TSLPR has been reported by several groups (2).
"Blinding" of the fieldworkers to the group assignment was not an option; however, all measurements except anthropometry were to a large extent automated (for example, biochemical assays, blood pressure, and arterial stiffness), thus reducing the possibility of bias.
For example, biochemical and mutational analyses of Cbes1865 (GH9 CBM CBM CBM GH5) showed that after deletion of the GH9 module, the truncated protein had an increased apparent Kcat value 2- to 3-fold higher on several mannan substrates, including locust bean gum, guar gum, and konjac glucomannan [ 20].
For example, biochemical techniques such as Western blotting give a measure of total phosphoprotein present in a particular sample that takes into account both the percentage of the population that is positive as well as the level of expression in that population.
