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In extreme cases - for example, experiments using genetically encoded reporters to study the morphologies of very large CNS neurons - the efficiency of recombination must be lowered to fewer than 20 cells per mouse [4].
For example, experiments using luciferase or GFP methods could be confirmed and generalised using our universally applicable PCR approach.
All these hypotheses could be addressed in the medaka system; for example, experiments using various tank mates, the eyeless or xanthophore mutants other than ci [ 35, 36], diet (carotenoid) restriction, or SLR knockout.
For example, experiments using mixed-species cDNA microarrays required raising expression fold-change thresholds to a level that limited the number of genes whose expression can be confidently measured [ 12].
For example, experiments using gene therapy approaches to restore SMN1 expression have yielded impressive amelioration in neuromuscular dysfunction and large increases in the lifespan of mice with SMA [ 11- 14].
For example, experiments using research-laboratory-scale quantities of Human Immunodeficiency Virus, a blood-borne virus, can be performed in a biosafety level-2, rather than biosafety level-3 facility (http://www.cdc.gov/biosafety).
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An example of experiments using real-world scenes as reference are the ones conducted by Čadik [14] that evaluated 14 TMOs in three different scenes.
For example, in experiments using mitotracker to visualize transferred sperm, we also observed that mating frequencies were similar for wild-type and comp-1 males.
To date, examples of experiments using microarrays to evaluate changes in gene expression in mammalian species other than humans and rodent species are lacking, primarily due to the limited availability of arrays.
Such an animal model, for example, would permit experiments using genetically uniform subjects within a controlled environment, as well as high-throughput screening of potential therapeutic agents.
For example, in vivo experiments using murine arthritic models that employed intra-articular adenoviral gene transfer of dominant negative IκB kinase β [ 8] or super repressor IκBα [ 9], or alternatively intra-articular injection of NF-κB decoy oligonucleotides [ 9, 10] demonstrated decreased severity of joint swelling.
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