Five widely used AML cell lines representing various AML subtypes were transplanted and expanded into highly immunodeficient non-obese diabetic/LtSz-severe combined immunodeficiency IL2Rγcnull mice (for example, cell line-derived xenografts).
For example, cell lines that overexpress PLCγ have a malignant phenotype [ 1].
For example, cell lines established from plasmacytoma are not abundant with less than 10 cell lines with evident plasmacytoma available at ATCC (https://www.atcc.org/).
For example, cell lines from genetically unrelated individuals representing different organs and tissues may significantly help in the functional validation of SNPs identified in clinical genetic-phenotypic association studies [ 162].
Finally, the results of these studies suggest that we should re-evaluate some of the cancer research models, for example, cell lines of metastatic origin (which largely exclude genomic evolution at distant sites) and preclinical models (which usually rely on the efficacy of drugs on primary tumors).
Figure 3 showed four examples, cell line KASUMI-1 with AML1-ETO, K-562 with BCR-ABL b3a2 splice variant, AML patient with PML-RARA S-form, and ALL patient with E2A-PBX1(I), respectively.
Users can select horizontal and vertical grouping categories, for example cell-lines tested or treatments applied, which will then be reflected by different facets of a bar plot.
The differences may be the result of many different aspects, for example, tumour cell line and size, as well as administered dose and specific activity of the conjugates.
The second example involves cell line K1, which shares the complex cytogenetic markers of cell line GLAG-66.
For example, the cell line OV2295 formed semi-compact spheroids, compared to the aggregates in TOV2295(R), possibly reflecting differences in cell to cell adhesion.
