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This has been done by pyrosequencing [ 24], microarray hybridization [ 25], and more recently by examining read depth of SNPs using next generation sequence data (Pool-seq) [ 26].
When examining read alignments relative to their position along the length of genes, a bias was observed toward reads mapping to the 5′-most portion of the gene regardless of their beginning base (Fig. 3d).
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In order to reduce memory requirement, each time Pindel loads one chromosome into memory and scan the entire read alignment file, examining reads associated with the current chromosome.
This set of total reads can be used together for the initial de novo genome assembly, but each individual's genotype also can be determined by examining reads with the appropriate barcode.
As further evidence for contamination of the GRO-seq data with RNA not originating from nuclear run-on experiments, we examined read distributions along various non-coding RNA families.
Deakin et al. examined read errors within libraries of small numbers of barcode sequences.
In addition to compiling new junction evidence for structural variants as detailed above, breseq includes a step where it examines read coverage for evidence of deleted chromosomal regions.
The geneBody_coverage.py script within the RseqC package [ 59] was used to examine read coverage across gene models and test for 5′ and 3′ bias in transcript coverage.
Here, we examine read coverage on a per-locus basis in the De Bruijn graph and, once a particular locus has sufficient coverage, call errors or trim bases for all following reads belonging to that graph locus.
To analyze RNA polymerase II activity within genes, we examined read depth along a set of 2,530 transcript models of genes that were transcriptionally active in both the NRO and total RNA libraries.
And yet, all of this focus on the test — which examines reading comprehension, math skills, the ability to reason logically — suggests a myopia of its own.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com