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This work examines the binding of 15 different VH3 IgGs and their corresponding F ab' 2 fragments to two different protein A chromatography resins: MabSelect®, which utilizes a recombinant protein A ligand, and MabSelect SuRe® (SuRe), which utilizes a tetrameric Z domain ligand.
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In the context of the full-length protein, we examined the binding of WT MeCP2 and MeCP2-R306C and found that both bind DNA with equal affinity in this in vitro assay.
The model system was further validated by examining the binding of one of the Opa proteins found in Neisseria and known to bind to human CEACAM1.
Employing a solid phase microplate-binding assay, we examined the binding of each viral ligand to wild type gC1qR and 11 gC1qR deletion mutants.
In the current studies we examined the binding of endogenous ligands, artificial ligands, and potent endocrine-disrupting chemicals to wild-type and Asp351 mutants of the human estrogen receptor-α ligand-binding domain.
With this goal in mind, we examined the binding of kanamycin A and four derivatives (the products of enzymic turnovers of kanamycin A by aminoglycoside-modifying enzymes) to a 27 nucleotide RNA representing the bacterial ribosomal A site.
We recently developed non-secosteroidal VDR ligands based on a carbon-containing boron cluster, 1,12-dicarba-closo-dodecaborane (p-carborane), and examined the binding of one of them to VDR by means of crystallographic analysis.
Here, we have examined the binding of CD8 to several variants of the HLA A2-restricted telomerase540 548 antigen (ILAKFLHWL) and the HLA A2-restricted NY-ESO-1157 165 aNY-ESO-1157 165QC) NY-ESO-1157 165eir cognantigenSLLMWITQCsthatt affinities and kinetics.
Next, we examined the binding of Sdo1p mutants to the 60S subunit by co-sedimentation assay.
We examined the binding of VWF to collagen 4 in vitro and extended this characterization to a murine model of defective VWF-collagen 4 interactions.
To determine whether glucose or insulin exert direct effects on the PL receptor in ovine fetal tissues, we examined the binding of radiolabeled oPL to ovine fetal hepatocytes and fibroblasts in culture.
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