To examine matrix metalloproteinase-3 (MMP-3) expression in human stromal cell culture after P stimulation and the effect of conditioned medium from human embryo-epithelial cells coculture on its expression and activity.
Initial experiments, examining matrix effects, involved replicate injections of a perchlorate-free local drinking water that was spiked with 1 and 5 µg/L perchlorate using injection volumes between 500 and 1000 µL.
Moreover, further research may examine matrix maturation during self-assembly of differentiated embryonic stem cells [54], which more closely resemble the phenotypic state of cells during the mesenchymal condensation phase of native articular cartilage development.
We also examined matrix M for degeneracies.
Similar results have been obtained in experiments examining matrix rigidity and differentiation of bronchial fibroblasts to a myofibroblast phenotype [ 56].
To examine matrix proteolysis, we plated cells on fluorescent gelatin and probed for the actin- and TKS5-enriched, matrix-degrading organelles called invadopodia (Murphy & Courtneidge, 2011).
So far, none of these previously mentioned studies examined matrix remodelling simultaneously in different compartments of the lung and in both moderate and severe COPD patients.
To determine the effect of mineral fibers and cigarette smoke on remodeling of lung tissues, we examined matrix metalloproteinase-1 (MMP-1), MMP-2, tissue inhibitors of metalloproteinase-1 (TIMP-1), TIMP-2, and types I and IV collagen mRNA levels from rat lungs exposed to mineral fibers and/or cigarette smoke in vivo.
In more detail, all ROC162 curves in Figure 1 are very alike in the initial regions and this is expected as all the examined matrices perform similarly when aligning highly similar proteins.
The other examined matrices, the PAMs and the BLOSUMs, were deemed inapplicable for modeling the code evolution, essentially, because these matrices have been derived from comparisons of protein sequences that are encoded by the standard code, and so cannot be independent of that code; as shown above, this applies to PAM74-100 as well as to the classic PAM matrices [ 48].
