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The topology of the electron density was examined using the program package AIMAll [20] with all the default options.
The potential for horizontal gene transfer in the genome of Eklund 17B (NRP) was examined using the program AlienHunter [ 26] to identify particularly high scoring regions in addition to visualisation of whole genome comparisons.
To functionally analyze these clusters, the sets of genes from each cluster were examined using the program DAVID, which identifies overrepresented functional groups among the genes in each cluster, as compared to all the genes represented on the array platform (see Methods; Additional file 7; DAVID is the Database for Annotation, Visualization and Integrated Discovery [ 22]).
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Furthermore, for the first time, the pharmacological and biological activities of the various polyhydroquinoline derivatives were computationally examined using the PASS program.
Natural selection of specific amino acid sites in Gag, Nef and Pol were examined using the codeml program from the PAML package (version 3.13) [49].
The DNA alignments and deduced amino acid sequences were examined using the BLAST program (National Center for Biotechnology Information, USA).
The cells were examined using the Fluoview program (Olympus Optical Co., Germany).
The function, biological processes and pathways of these genes were then examined using the GeneSpring program.
Both orthogonal (varimax) and oblique (direct-oblimin) rotations were examined using the FACTOR program [ 29].
Graphical layouts of individual clusters were examined using the SeqGrapheR program (Novák et al., 2010).
Gene lists from Affymetrix results were examined using the EASE program, accessible via http://david.abcc.ncifcrf.gov/.abcc.ncifcrf.gov/
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