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In order to find out whether Wolbachia regulates the AeCHD genes during infection, RT-qPCR was performed to examine the transcript levels of all the three AeCHDs with and without Wolbachia infection in whole mosquitoes.
The purpose of the present study was to simultaneously examine the transcript levels of a large number of MMPs (-3, -8, -9, -13 and -14) and ADAMTS-4 and to investigate their correlation with the clinicopathological profile of patients suffering from tuberculous intervertebral discs.
Previously, we have performed a microarray analysis to examine the transcript levels of >23000 genes using the Affymetrix U133A Genechip [24], and the data have been made available to the general public (GEO, http://www.ncbi.nlm.nih.gov/gds/ with GEO series accession number GSE2513).
It would be interesting to examine the transcript levels of these enzymes in Bitis or Dendroaspis venoms by comparison.
SAGE allowed us to examine the transcript levels for a few thousand genes (typically, 4000 7000 per library) in one experiment.
Quantitative PCR array technology was exploited to examine the transcript levels of 84 AM genes in CD34+ pools from CR AML patients, exhibiting a chemoresistant or a chemosensitive phenotype after in vitro treatment, and control donors.
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In order to examine the transcripts levels between olfactory and non-factory tissues, amplification of a "house-keeping" gene encoding actin was used as control to check the integrity of each cDNA preparation.
In order to examine the transcripts levels between olfactory and non-olfactory tissues, specific primers of a "housekeeping" gene encoding ribosomal protein L8 (CquiRpL8) were used as control to check the integrity of each cDNA preparation.
To determine whether decreased Bcl-2 expression and increased Bax expression were caused by gene transcription, we examined the transcript levels of Bcl-2 and Bax using semi-quantitative RT-PCR.
Considering the virus blocking effect of Wolbachia in Ae. aegypti mosquitoes, we examined the transcript levels of AeCHD7 in the context of mosquito-DENV interaction.
In addition, we examined the transcript levels of NIA1, NiR, NRT2.1, HHO1, and HRS1 which are responsive to nitrate in a short time.
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