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We wanted to examine the functional consequences of the promoting cPERK autophosphorylation (Figure 4D).
Having determined that fusion of Tec1 and Ubc9 enhances sumoylation of Tec1 at Lys54, we sought to examine the functional consequences of increasing sumoylation of Tec1.
Conceivably, once the sumoylation site on Ste12 is identified, the UFDS approach could also be applied to examine the functional consequences of Ste12 sumoylation.
To examine the functional consequences of the blockade of calpain activity on the UCB effects, slices were co-exposure to UCB (10 µM) and leupeptin or calpeptin.
The marked sensitivity of PIK3CA-mutated and/or HER2-overexpressing breast cancer cells to AKTi-1/2 allowed us to examine the functional consequences of Akt blockade in these cells.
Future work should be directed to identify Ste12 sumoylation site(s) and examine the functional consequences of inhibiting Ste12 sumoylation (via Lys-to-Arg mutation of the sumoylation site).
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To examine the functional consequence of H5N1 NS1-mediated inhibition of IFN-inducible STAT phosphorylation, we examined STAT-DNA binding, using electrophoretic mobility shift assay (EMSA) studies.
We then used Rab27a/Rab27b double knockout (DKO) mice to examine the functional consequence of loss of Rab27 proteins in the murine lung.
To examine the functional consequence of caspase-mediated GFAP proteolysis at VELD, MCF7 cells were transiently transfected with either wild-type GFAP or caspase cleavage-resistant D225E mutant.
To examine the functional consequence of elevated MMP28 expression in gastric cancer cells, His-tagged MMP28 was overexpressed in N87 gastric cancer cells, which exhibit a low endogenous level of MMP28.
Here, we examined the functional consequences of reelin secretion blockade on the subunit composition of NMDARs.
More suggestions(17)
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