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Here we examine the expression profile of paclitaxel biosynthetic pathway genes by RNA gel blot analysis and RT-PCR in the Taxus cuspidata cell line P991 and compare with taxane metabolite levels.
As kallikrein-related peptidase 12 (KLK12) has been implicated in the cancer progression and alternative splicing plays significant role in this disease, the aim of this study was to examine the expression profile and the clinical impact of the KLK12 splice variants in breast cancer.
Therefore it is essential to examine the expression profile and properties of individual MIC and ULBP/RAET molecules to get a fuller understanding of NKG2D function.
To examine the expression profile of RLK902 in detail, in situ hybridization analysis was performed and promoter and protein fusions constructed.
To examine the expression profile of Sun1η during mammalian spermatogenesis, we next performed RT-PCR on total RNA of pubertal testes [46], [47].
To examine the expression profile of RKL1 we constructed the RKL1 GUS reporter fusing a 2548 bp genomic DNA fragment upstream of the coding region to GUS.
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The generated reads were then mapped to the genome sequence to examine the expression profiles of the chemosensory genes in these four tissues.
K-means clustering analysis was further performed to examine the expression profiles across different internode segments (Fig. 4C).
Objective: To examine the expression profiles of luteinized granulosa cells isolated from women with normal or diminished ovarian reserve undergoing in vitro fertilization.
To examine the expression profiles of caveolin-1, integrin α7, CTR, emerin and lamin A/C, isolated myofibres were cultured for 48 hrs, before being fixed and immunostained for MyoD and the relevant marker (Figure 3).
The significant perturbation observed in TP1 motivated us to examine the expression profiles of individual genes (and combinations) in this pathway and to explore their application in prediction models.
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