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Amplification was seen just as frequently in tumours that do not express eEF1A2; it would be of interest to examine the expression levels of other genes within this amplicon.
The aim of this study is to investigate the role of TSG101 in human ovarian cancer development, to examine the expression levels of TSG101 in ovarian carcinomas, and to correlate the results with clinicopathologic variables and survival.
To examine the expression levels of the rice genes, PCR was performed with 4 μl of a diluted cDNA reaction mixture in a final volume of 20 μl using Taq DNA polymerase (New England Biolabs).
To test if the changes in tiller number among these plants were related to the expression of rice SPL14 (Luo et al. 2012), D53 (Zhou et al. 2013) and TB1 (Choi et al. 2012) that are known to be involved in regulation of tillering, we conducted qRT-PCR to examine the expression levels of these genes in the null, heterozygous and homozygous plants derived from the OsCKX2 overexpression line.
We further performed qRT-PCR to examine the expression levels of randomly selected miR-223.
Attenuation of bone density in TH mice led us to examine the expression levels of osteoblast- and osteoclast-mediated gene markers in bone marrow cells.
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In a small test at the beginning of the present study, western blotting and qPCR were performed to examine the expression level of GNA13 in several paired HCC and adjacent non-neoplastic tissues.
We used qRT-PCR analysis to examine the expression level of OsSND2 in these transgenic plants.
Immunohistochemistry (IHC) was used to examine the expression level of Ubc9.
Next, we examined the expression levels of adherens junction components in pRb-expressing osteoblasts stably expressing either dnCdk5 or RacV12.
We examined the expression levels of the nuclear-encoded TFAM protein since its levels are directly proportional to mt-DNA copy number23.
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