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We used qRT-PCR analysis to examine the expression level of OsSND2 in these transgenic plants.
To examine the expression level of PGC-1α in rat VSMCs, total RNA was isolated using RNeasy kit (Qiagen, Hilden, Germany).
In this report, we performed high-throughput miRNA profiling to examine the expression level of miRNAs in pAGMK cells and in VERO cells at non-tumorigenic and tumorigenic stages of neoplastic development.
To examine the expression level of Mieap and to induce MALM, or MIV by mitochondrial damage in response to genotoxic stresses, in all the figures and supplementary figures, the cells were γ-irradiated at 60 Gy using a 60Co source.
Therefore, to provide a molecular basis for such changes in ion channel function, it is necessary to examine the expression level of relevant ion channel subunit proteins in cardiac myocytes.
To examine the expression level of p-MK2 in mouse oocytes at different stages of meiotic maturation, samples were collected after oocytes had been cultured for 0, 4, 8, 9.5, and 12 h, corresponding to germinal vesicle (GV), prometaphase I (Pro-MI), metaphase I (MI), telophase I (TI), and metaphase II (MII) stages, respectively.
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We further performed qRT-PCR to examine the expression levels of randomly selected miR-223.
qPCR was used to examine the expression levels of duox in 6- and 24-hpi larvae.
To examine this hypothesis, we examine the expression levels of WNK1 and WNK4 in the kidneys of WNK3 knockout mice.
Real-time qPCR was performed on the cDNA to examine the expression levels of mRNAs and miRNAs.
We then performed FACS analysis to examine the expression levels of two HIV-1 co-receptors, CCR5 and CXCR4.
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