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We used the GHMM C++ library [29] (python bindings) to examine target sequences with our COUP-TF HMM parameters and the Viterbi algorithm [28].
To examine target genes of H3K9 methylation dependent silencing by either G9a or SUV39H1, we analyzed genes up-regulated after inhibition of G9a or SUV39H1 using oligonucleotide microarrays.
We also used LNA-anti-miRNAs (see below) along with scrambled controls to examine target gene expression.
Using the Mayo Clinic General Clinical Research Center we utilized rt-PCR to examine target genes and a housekeeping gene (18S) for 29 of the Mayo samples for which at least 1 μg of RNA was available for cDNA synthesis.
The primary aim of this investigation was to examine target labelling methodologies that could be used for our custom miRNA microarray that did not require labour intensive steps such as gel purification, were sensitive and specific, useable at high-throughput and more affordable than multiplex RT-PCR assay.
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Larger studies are warranted to examine targeted treatment elements and individual difference factors to enhance cessation outcomes in African-American smokers.
Bevan and Hood (2006) examine targets in the English public health care system.
Our current implementation of Think3d! will further examine targeted math improvements both relative to other problem types and relative to control classrooms who have not had embodied spatial training.
The UN Department of Economic and Social Affairs (DESA) list of major UN summits and conferences from 2001 to 2012 was utilised to examine targets.
Thus, we used an ex vivo approach with human skin explants to examine targeting of two important C-type lectin receptors, Langerin and DEC-205.
We examined target Ags of BNP Abs on PBLk by immunoprecipitation.
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CEO of Professional Science Editing for Scientists @ prosciediting.com