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The current exploratory study sought to examine sequence variations in the superoxide dismutase 1 (SOD1) and 2 (SOD2) genes in patients with myelomeningocele and to identify variants altering risk for myelomeningocele.
To examine sequence conservation across the 2 kb 5' flanking regions, a TCOFFEE multiple sequence alignment was prepared (Figure S1).
Algorithms may examine local sequence effect on site accessibility, or examine sequence content for particular nucleotides [27], [30], [33].
To examine sequence relationships within the KAP-β family across the eukaryotes, we performed a semi-automated search of a panel of predicted proteomes.
SNP based genotyping has been recently used for M. tuberculosis but this analysis does not examine sequence of nucleotide stretches but rather point mutation detected by an hybridization-based method SnaPshot [21].
The goal is to examine sequence co-variance and distinguish between correlations of different origin.
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To understand the solution to this dilemma, it pays to examine sequences: an important aspect of pattern application that is often overlooked.
In the future it will be important to devise approaches to examine sequence-level, epigenetic and large-scale changes together and relate these to clinical features to form a complete and integrated picture.
To examine sequencing accuracy, TA cloning was performed.
To examine sequencing variations, cDNA library replicates of each condition were constructed.
We used local haplotyping analysis to examine sequencing reads that span 2 or 3 adjacent heterozygous SNPs.
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