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To dissect the point of Calcineurin action during merozoite invasion, we developed an in vitro assay to examine processing of the micronemal protein, PbAMA1 (based on Singh et al., 2014), and also to inspect merozoite attachment to the erythrocyte (based on the accompanying manuscript, Paul et al., 2015).
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Follow-up analyses examined processing speed subtest performance and relations between processing speed and white matter microstructure (as measured with diffusion tensor imaging [DTI] in a subset of these participants).
In addition, we examined processing of the membrane-bound C-terminal fragment of Sema4C that is produced after ectodomain shedding by appending an HA epitope tag to the C-terminus.
To ensure that WRN can digest the strands in the context of the D-loop we also examined processing in the absence of branch migration (ATPγS and no ATP).
A prototypical candidate for examining processing specificity is that of interoceptive ability.
To further explore how FREM1 might affect PDGFC, we next examined processing of PDGFC.
Examined processing modes were flexible relational and rigid non-relational (single item) encoding and retrieval.
Examined processing modes were single item versus relational encoding with only relational encoding hypothesized to depend on hippocampus.
At the molecular level, Fas-induced proapoptotic signaling was assayed on day 1 of culture by examining processing of pro-caspase 8 and 3 that indicates their activation.
Next, we examined processing of caspase-9 in DAOY M2 cells, transfected with siRNAs for 48 h and treated for 72 h with cisplatin and etoposide.
To further examine the functionality of the lysosomes, we examined processing of Cathepsin-L and found no significant difference in Cathepsin-L processing in VCP-RNAi animals or animals treated with DBeQ.
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