Suture line was examined for integrity and an additional stitch was applied when necessary.
The resulting RNAs were purified by LiCl precipitation and examined for integrity by PAGE.
Posterior intestine sections were examined for integrity of the intestinal mucosa and the presence of any inflammatory response.
Each sam-ple was measured in triplicate, and data points were examined for integrity by analysis of the amplification plot.
Each sample was measured in triplicate, and data points were examined for integrity by analysis of the amplification plot.
Sequences were examined for integrity using the ABIPrism EditView ABI Automated DNA Sequence Viewer (version 1.0.1) viewer programme and analysed for mutation using DNAStar Lasergene (v6.0) sequence analysis software.
After data had been recorded for 4 h, the bilayers were further examined for integrity by adding, to both sides of the bilayer, gramicidin A (gA), a peptide known to form well-defined ion channels.
Total RNA was extracted using an Easy-Blue total RNA extraction kit (Intron Biotech, Sungnam, Korea), purified using Qiagen RNeasy Mini Kits (Qiagen, Basel, Switzerland), and examined for integrity using an Agilent 2100 Bioanalyzer (Ambion, Austin, TX, USA).
Adjacent sections were hematoxilin-eosin stained and examined for tissue integrity.
Adjacent sections were hematoxylin-eosin stained (Laborclin, Pinhais, Brazil) and examined for tissue integrity.
The tests were stored in a cool and dry location for the duration of the trial until use, and each package was examined for the integrity of the desiccant.
